http://hdl.handle.net/10311/18 2026-07-11T06:35:47Z 2026-07-11T06:35:47Z Rantshabeng, Patricia Kasvosve, Ishmael Ndlovu, Andrew Gaseitsiwe, Simani Moyo, Sikhulile http://hdl.handle.net/10311/2249 2021-12-18T00:00:42Z 2019-01-30T00:00:00Z

Prevalence of high-risk human papilioma virus in women with high-grade squamous cell intraepithelial lesions in Botswana using abbott real time HPV assay Rantshabeng, Patricia; Kasvosve, Ishmael; Ndlovu, Andrew; Gaseitsiwe, Simani; Moyo, Sikhulile Background: High-risk human papillomavirus (HR-HPV) has been demonstrated to be the necessary cause of cervical carcinoma. High-risk HPV detection has a prognostic significance for the women who are at increased risk of disease progression. HPV genotyping in cervical cancer precursor lesions is crucial for prevention and management of cervical cancer. This study was designed to investigate the distribution of HR-HPV genotypes among a group of patients with high-grade squamous intraepithelial lesions and higher, of the cervix, in Botswana. Materials and methods: 185-archived residual formalin-fixed paraffin-embedded cervical biopsies collected between the years, 2006 and 2008 were studied. These tissues were diagnosed with HSIL (n = 146) and squamous cell carcinoma (n = 39). DNA was extracted using the Abbott m2000 analy-ser (Abbott Laboratories, Illinois) using reagents provided by the manufacturer. HPV geno-typing was done using the Abbott Real Time HR-HPV PCR, which qualitatively detects 14 HR-HPV (HR-HPV (reported as HPV 16, 18 and other HPV). Results: DNA was successfully extracted from 162/185 (87.6%) tissues as indicated by a positive β -globin test. 132/162 (82%) tested positive for HR-HPV The HPV 16 prevalence was 50% (66/132), HPV 18 at 15.2% (20/132) and other Group 1 HR-HPV plus HPV 66 and 68 had a prevalence of 56.1% (74/132). Other HR-HPV types were common in HSIL than in carci-noma, while HPV 16 was more prevalent in carcinomas than other HR-HPV genotypes . Conclusion: In this study, HPV 16 and other HR-HPV genotypes were commonly associated with HSIL but HPV 18 was uncommon among Botswana women. Our data highlights the need for mul-tivalent HPV vaccines with cross coverage for other high risk HPV other than HPV 16 and 18.

2019-01-30T00:00:00Z Adewole, I.F. Abauleth, Y.R. Adoubi, I. Amorissani, F. Anorlu, R.I. Awolude, O.A. Botha, H. Byamugisha, J.K. Cisse, L. Diop, M. Doh, S. Fabamwo, A.O. Gahouma, D. Galadanci, H.S. Githanga, D. Magure, T.M. Mabogunje, C. Mbuthia, J. Muchiri, L.w. Ndiaye, O. Nyakabau, A.M. Ojwang, S.B.O. Ramogola-Masire, D. Sekyere, O. Smith, T.H. Taulo, F.O.G. Wewege, A. Wiredu, E. Yarosh, O. http://hdl.handle.net/10311/2181 2021-10-08T00:00:47Z 2013-01-01T00:00:00Z

Consensus recommendations for the prevention of cervical cancer in sub-Saharan Africa Adewole, I.F.; Abauleth, Y.R.; Adoubi, I.; Amorissani, F.; Anorlu, R.I.; Awolude, O.A.; Botha, H.; Byamugisha, J.K.; Cisse, L.; Diop, M.; Doh, S.; Fabamwo, A.O.; Gahouma, D.; Galadanci, H.S.; Githanga, D.; Magure, T.M.; Mabogunje, C.; Mbuthia, J.; Muchiri, L.w.; Ndiaye, O.; Nyakabau, A.M.; Ojwang, S.B.O.; Ramogola-Masire, D.; Sekyere, O.; Smith, T.H.; Taulo, F.O.G.; Wewege, A.; Wiredu, E.; Yarosh, O. Cervical cancer is the second most common cancer and the leading cause of cancer-related death in women in sub-Saharan Africa. It is estimated that more than 200 million females older than 15 years are at risk in this region. This paper highlights the current burden of cervical cancer in sub-Saharan Africa, reviews the latest clinical data on primary prevention, outlines challenges in the region, and offers potential solutions to these barriers. Based on these factors, clinical recommendations for the prevention of cervical cancer from the sub-Saharan African Cervical Cancer Working Group expert panel are presented. Editorial assistance was provided by Dr Ian Seymour and Dr Tim Blackstock from Wells Healthcare Communications, funded with support from GSK. The authors take full responsibility for the content of this manuscript.

2013-01-01T00:00:00Z DiAngi, Yumi Taylor Panozzo, Catherine A. Ramogola-Masire, Doreen Steenhoff, Andrew P. Brewer, Noel T. http://hdl.handle.net/10311/2178 2021-10-07T00:00:46Z 2011-10-25T00:00:00Z

A cross-sectional study of HPV vaccine acceptability in Gaborone, Botswana DiAngi, Yumi Taylor; Panozzo, Catherine A.; Ramogola-Masire, Doreen; Steenhoff, Andrew P.; Brewer, Noel T. Background Cervical cancer is the most common cancer among women in Botswana and elsewhere in Sub-Saharan Africa. We sought to examine whether HPV vaccine is acceptable among parents in Botswana, which recently licensed the vaccine to prevent cervical cancer. Methods and Findings We conducted a cross-sectional survey in 2009, around the time the vaccine was first licensed, with adults recruited in general medicine and HIV clinics in Gaborone, the capital of Botswana. Although only 9% (32/376) of respondents had heard of HPV vaccine prior to the survey, 88% (329/376) said they definitely will have their adolescent daughters receive HPV vaccine. Most respondents would get the vaccine for their daughters at a public or community clinic (42%) or a gynecology or obstetrician's office (39%), and 74% would get it for a daughter if it were available at her school. Respondents were more likely to say that they definitely will get HPV vaccine for their daughters if they had less education (OR = 0.20, 95% CI = 0.07–0.58) or lived more than 30 kilometers from the capital, Gaborone (OR = 2.29, 95% CI = 1.06–4.93). Other correlates of acceptability were expecting to be involved in the decision to get HPV vaccine, thinking the vaccine would be hard to obtain, and perceiving greater severity of HPV-related diseases. Conclusions HPV vaccination of adolescent girls would be highly acceptable if the vaccine became widely available to the daughters of healthcare-seeking parents in Gaborone, Botswana. Potential HPV vaccination campaigns should provide more information about HPV and the vaccine as well as work to minimize barriers.

2011-10-25T00:00:00Z Madzimbamuto, Farai D. Ray, Sunanda C. Mogobe, Keitshokile D. Ramogola-Masire, Doreen Phillips, Raina Haverkamp, Miriam Mokotedi, Mosidi Motana, Mpho http://hdl.handle.net/10311/2177 2021-10-07T00:00:35Z 2014-07-16T00:00:00Z

A root-cause analysis of maternal deaths in Botswana: towards developing a culture of patient safety and quality improvement Madzimbamuto, Farai D.; Ray, Sunanda C.; Mogobe, Keitshokile D.; Ramogola-Masire, Doreen; Phillips, Raina; Haverkamp, Miriam; Mokotedi, Mosidi; Motana, Mpho Background: In 2007, 95% of women in Botswana delivered in health facilities with 73% attending at least 4 antenatal care visits. HIV-prevalence in pregnant women was 28.7%. The maternal mortality ratio in 2010 was 163 deaths per 100 000 live births versus the government target of 130 for that year, indicating that the Millennium Development Goal 5 was unlikely to be met. A root-cause analysis was carried out with the aim of determining the underlying causes of maternal deaths reported in 2010, to categorise contributory factors and to prioritise appropriate interventions based on the identified causes, to prevent further deaths. Methods: Case-notes for maternal deaths were reviewed by a panel of five clinicians, initially independently then discussed together to achieve consensus on assigning contributory factors, cause of death and whether each death was avoidable or not at presentation to hospital. Factors contributing to maternal deaths were categorised into organisational/management, personnel, technology/equipment/supplies, environment and barriers to accessing healthcare. Results: Fifty-six case notes were available for review from 82 deaths notified in 2010, with 0–4 contributory factors in 19 deaths, 5–9 in 27deaths and 9–14 in nine. The cause of death in one case was not ascertainable since the notes were incomplete. The high number of contributory factors demonstrates poor quality of care even where deaths were not avoidable: 14/23 (61%) of direct deaths were considered avoidable compared to 12/32 (38%) indirect deaths. Highest ranking categories were: failure to recognise seriousness of patients’ condition (71% of cases); lack of knowledge (67%); failure to follow recommended practice (53%); lack of or failure to implement policies, protocols and guidelines (44%); and poor organisational arrangements (35%). Half the deaths had some barrier to accessing health services. Conclusions: Root-cause analysis demonstrates the interactions between patients, health professionals and health system in generating adverse outcomes for patients. The lessons provided indicate where training of undergraduate and postgraduate medical, midwifery and nursing students need to be intensified, with emphasis on evidence-based practice and adherence to protocols. Action plans and interventions aimed at changing the circumstances that led to maternal deaths can be implemented and re-evaluated.

2014-07-16T00:00:00Z