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http://hdl.handle.net/10311/484| Title: | Investigating evidence for UV-B induced mutagenesis in dimorphotheca sinuata using the 18S rDNA and rbcL gene sequences |
| Authors: | Mpoloka, S.W. |
| Keywords: | UV-B DNA damage Recombination DNA repair Pyrimidine dimers Mutagenesis |
| Issue Date: | 2008 |
| Publisher: | American-Eurasian Network for Scientific Information. http://www.aensi.org/aeb_online.html |
| Citation: | Mpoloka S. W. (2008). Investigating evidence for UV-B induced mutagenesis in dimorphotheca sinuata using the 18S rDNA and rbcL gene sequences, Advances in Environmental Biology, vol. 2 (2): pp. 81-88 |
| Abstract: | The nuclear 18S rDNA and chloroplast encoded rbcL gene sequences were used to investigate the evidence of residual UV-B induced mutagenesis in Dimorphotheca sinuata plants. The probes were generated by the polymerase chain reaction and labelled with a non-radioactive Digoxigenin label and used in Southern hybridization studies. High levels of variability in the 18S rDNA gene were found, pointing to genome rearrangements and possibly genome instability due to UV-B effects. |
| URI: | http://hdl.handle.net/10311/484 |
| ISSN: | 1995-0756 |
| Appears in Collections: | Research articles (Dept of Biological Sciences) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Investigating evidence.pdf | 1.2 MB | Adobe PDF |  View/Open |
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