Show simple item record

dc.contributor.authorLoeto, D.
dc.contributor.authorWale, K.
dc.contributor.authorKhare, K.B.
dc.contributor.authorCoetzee, T.
dc.contributor.authorBaruti, K.
dc.date.accessioned2016-11-01T14:14:21Z
dc.date.available2016-11-01T14:14:21Z
dc.date.issued2016
dc.identifier.citationLoeto, D. et al. (2016) Mating type determination of cryptococcus neoformans isolates obtained from faecal samples of veterinary animals in Botswana. CIBTech Journal of Microbiology, Vol. 5 No. 3, pp. 16-22en_US
dc.identifier.issn2319-3867 (Online)
dc.identifier.urihttp://hdl.handle.net/10311/1547
dc.description.abstractCryptococcus neoformans/gattii species complex cause cryptococcosis, a fatal condition in susceptible humans and animals. This paper aims to find the association of C. neoformans with veterinary excreta, and to carry out a mating type determination of the isolated strains. Two hundred sixty seven veterinary excreta samples from cattle, goats and poultry (chickens) were analysed for the presence of C. neoformans. Morphological and biochemical characteristics were used to identify C. neformans, and mating type-specific PCR amplification was utilized to determine the mating type of the isolates. A total of 72 (26.9%) isolates were found positive for C. neoformans, mostly isolated from cattle (14.9%), followed by poultry (9.4%) and goats (2.6%). However, the isolation rates of the organism from veterinary animals were not statistically significant (p<0.05). Mating type analysis of the 72 positive isolates revealed the presence of MATα, MATα/MATa (hybrids) and MATa in cattle (31.9%, 4.2%, 19.4%)> poultry (19.4%, 2.8%, 12.5%)> and goats (8.3%; 0%, 1.4%), respectively. The frequency of isolation of different mating types was not influenced by the veterinary animals (p<0.05) or sampling location (p<0.05). The results of our study expand the ecological niche of C. neoformans in Botswana and highlight the need to mitigate the dissemination of the pathogen to the population at risk.en_US
dc.description.sponsorshipSymbols may not appear as in the original articleen_US
dc.language.isoenen_US
dc.publisherCentre for Info Bio Technology (CIBTech), http://www.cibtech.org/en_US
dc.subjectCryptococcus neoformansen_US
dc.subjectveterinary animalsen_US
dc.subjectmating typeen_US
dc.subjectMATαen_US
dc.subjectMATaen_US
dc.subjecthybrid genotypesen_US
dc.titleMating type determination of cryptococcus neoformans isolates obtained from faecal samples of veterinary animals in Botswanaen_US
dc.typePublished Articleen_US
dc.linkhttp://www.cibtech.org/J-Microbiology/PUBLICATIONS/2016/VOL-5-NO-3/03-CJM-004-KRISHNA-ARTICLE-MATING-BOTSWANA.pdfen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record